Attempt to write a read-only database - System.Data.SQLite

Make sure that the anonymous web user account (NETWORK SERVICE or maybe something else) under which your web application executes has read/write/create/delete access to the folder where the sqlite database resides.

The other answer give me an idea about the problem, but my solution was a bit differente. The problem was that the user did not have permissions to modify the folder and the .db file. So I give permission to the user Everyone (collective group for Authenticated Users and Guest). The permisions on NETWORK SERVICE (or similars) did not solve in my case.

Note: The IIS must be restarted, after the change, to load the change made.