Avoid Adding duplicate elements to a List C#

You can use Enumerable.Except to get distinct items from lines3 which is not in lines2:

lines2.AddRange(lines3.Except(lines2));

If lines2 contains all items from lines3 then nothing will be added. BTW internally Except uses Set<string> to get distinct items from second sequence and to verify those items present in first sequence. So, it's pretty fast.

Your this check:

if (!lines2.Contains(lines3.ToString()))

is invalid. You are checking if your lines2 contains System.String[] since lines3.ToString() will give you that. You need to check if item from lines3 exists in lines2 or not.

You can iterate each item in lines3 check if it exists in the lines2 and then add it. Something like.

foreach (string str in lines3)
{
    if (!lines2.Contains(str))
        lines2.Add(str);
}

Or if your lines2 is any empty list, then you can simply add the lines3 distinct values to the list like:

lines2.AddRange(lines3.Distinct());

then your lines2 will contain distinct values.

Use a HashSet<string> instead of a List<string>. It is prepared to perform a better performance because you don't need to provide checks for any items. The collection will manage it for you. That is the difference between a list and a set. For sample:

HashSet<string> set = new HashSet<string>();

set.Add("a");
set.Add("a");
set.Add("b");
set.Add("c");
set.Add("b");
set.Add("c");
set.Add("a");
set.Add("d");
set.Add("e");
set.Add("e");

var total = set.Count;

Total is 5 and the values are a, b, c, d, e.

The implemention of List<T> does not give you nativelly. You can do it, but you have to provide this control. For sample, this extension method:

public static class CollectionExtensions
{
    public static void AddItem<T>(this List<T> list, T item)
    {
       if (!list.Contains(item))
       {
          list.Add(item);
       }
    }
}

and use it:

var list = new List<string>();
list.AddItem(1);
list.AddItem(2);
list.AddItem(3);
list.AddItem(2);
list.AddItem(4);
list.AddItem(5);