Can I pass an argument to a VBScript (vbs file launched with cscript)?

Each argument passed via command line can be accessed with: Wscript.Arguments.Item(0) Where the zero is the argument number: ie, 0, 1, 2, 3 etc.

So in your code you could have:

strFolder = Wscript.Arguments.Item(0) 

Set FSO = CreateObject("Scripting.FileSystemObject")
Set File = FSO.OpenTextFile(strFolder, 2, True)
File.Write "testing"
File.Close
Set File = Nothing
Set FSO = Nothing
Set workFolder = Nothing

Using wscript.arguments.count, you can error trap in case someone doesn't enter the proper value, etc.

MS Technet examples

Inside of VBS you can access parameters with

Wscript.Arguments(0)
Wscript.Arguments(1)

and so on. The number of parameter:

Wscript.Arguments.Count

You can use WScript.Arguments to access the arguments passed to your script.

Calling the script:

cscript.exe test.vbs "C:\temp\"

Inside your script:

Set File = FSO.OpenTextFile(WScript.Arguments(0) &"\test.txt", 2, True)

Don't forget to check if there actually has been an argument passed to your script. You can do so by checking the Count property:

if WScript.Arguments.Count = 0 then
    WScript.Echo "Missing parameters"
end if

If your script is over after you close the file then there is no need to set the variables to Nothing. The resources will be cleaned up automatically when the cscript.exe process terminates. Setting a variable to Nothing usually is only necessary if you explicitly want to free resources during the execution of your script. In that case, you would set variables which contain a reference to a COM object to Nothing, which would release the COM object before your script terminates. This is just a short answer to your bonus question, you will find more information in these related questions:

Is there a need to set Objects to Nothing inside VBA Functions

When must I set a variable to “Nothing” in VB6?