Conditionally add items to a list when defining the list?

I'd just insert them afterwards; lists are mutable after all:

l = [
    HeadObj(1),
    HeadObj(2),
    BodyObj(1),
    BodyObj(2),
    BodyObj(3),
    FooterObj(1)
]
if USE_SEPARATORS:
    l.insert(2, Separator())
    l.insert(6, Separator())