CPU and Data alignment
Very little data "has" to be aligned. It's more that certain types of data may perform better or certain cpu operations require a certain data alignment.
First of all, let's say you're reading 4 bytes of data at a time. Let's also say that your CPU has a 32 bit data buss. Let's also say your data is stored at byte 2 in the system memory.
Now since you can load 4 bytes of data at once, it doesn't make too much sense to have your Address register to point to a single byte. By making your address register point to every 4 bytes you can manipulate 4 times the data. So in other words your CPU may only be able to read data starting at bytes 0, 4, 8, 12, 16, etc.
So here's the issue. If you want the data starting at byte 2 and you're reading 4 bytes, then half your data will be in address position 0 and the other half in position 1.
So basically you'd end up hitting the memory twice to read your one 4 byte data element. Some CPUs don't support this sort of operation (or force you to load and combine the two results manually).
Go here for more details: http://en.wikipedia.org/wiki/Data_structure_alignment
1.) Some architectures do not have this requirement at all, some encourage alignment (there is a speed penalty when accessing non-alignet data items), and some may enforce it strictly (misaligment causes a processor exception).
Many of todays popular architectures fall in the speed penalty category. The CPU designers had to make a trade between flexibility/performance and cost (silicon area/number of control signals required for bus cycles).
2.) What language, which architecture? Consult your compilers manual and/or the CPU architecture documentation.
3.) Again this is totally architecture dependent (some architectures may not permit access on byte-sized items at all, or have bus widths which are not even a multiple of 8 bits). So unless you are asking about a specific architecture you wont get any useful answers.
CPUs are word oriented, not byte oriented. In a simple CPU, memory is generally configured to return one word (32bits, 64bits, etc) per address strobe, where the bottom two (or more) address lines are generally don't-care bits.
Intel CPUs can perform accesses on non-word boundries for many instructions, however there is a performance penalty as internally the CPU performs two memory accesses and a math operation to load one word. If you are doing byte reads, no alignment applies.
Some CPUs (ARM, or Intel SSE instructions) require aligned memory and have undefined operation when doing unaligned accesses (or throw an exception). They save significant silicon space by not implementing the much more complicated load/store subsystem.
Alignment depends on the CPU word size (16, 32, 64bit) or in the case of SSE the SSE register size (128 bits).
For your last question, if you are loading a single data byte at a time there is no alignment restriction on most CPUs (some DSPs don't have byte level instructions, but its likely you won't run into one).