How to find the mime type of a file in python?

More reliable way than to use the mimetypes library would be to use the python-magic package.

import magic
m = magic.open(magic.MAGIC_MIME)
m.load()
m.file("/tmp/document.pdf")

This would be equivalent to using file(1).

On Django one could also make sure that the MIME type matches that of UploadedFile.content_type.

The python-magic method suggested by toivotuo is outdated. Python-magic's current trunk is at Github and based on the readme there, finding the MIME-type, is done like this.

# For MIME types
import magic
mime = magic.Magic(mime=True)
mime.from_file("testdata/test.pdf") # 'application/pdf'

The mimetypes module in the standard library will determine/guess the MIME type from a file extension.

If users are uploading files the HTTP post will contain the MIME type of the file alongside the data. For example, Django makes this data available as an attribute of the UploadedFile object.