How to make a query date in mongodb using pymongo?

Query conditions on ISODate attributes should use Python's datetime.datetime objects.

That is, don't format your dates as strings using the isoformat function, use them as they are.

@Joni is correct, you need to use datetime.

from datetime import datetime
from pymongo import Connection

# i have updated and included the complete code 
client = Connection('localhost', 27017)
db = client['database'] # your database name
inoshare = db['inoshare']


# convert your date string to datetime object
start = datetime(2014, 9, 24, 7, 51, 04)
end = datetime(2014, 9, 24, 7, 52, 04)

inoshare.find( {'id_no': 1, 'datahora': {'$lt': end, '$gte': start}, 'porta': 'A0'})
<pymongo.cursor.Cursor at 0x7f9aafd64a90>

inoshare.find_one( {'id_no': 1, 'datahora': {'$lt': end, '$gte': start}, 'porta': 'A0'})

{u'_id': ObjectId('5435be9ce7b9916e02ed2cb5'),
 u'datahora': datetime.datetime(2014, 9, 24, 7, 51, 5),
 u'id_no': 1.0,
 u'lab': u'2',
 u'porta': u'A0',
 u'sensor': u'1',
 u'valor': u'917'}

clearly I can successfully return results. Perhaps your data is corrupt, or you should post all your code for us to review