Accessing certain pixel RGB value in openCV

A piece of code is easier for people who have such problem. I share my code and you can use it directly. Please note that OpenCV store pixels as BGR.

cv::Mat vImage_; 

if(src_)
{
    cv::Vec3f vec_;

    for(int i = 0; i < vHeight_; i++)
        for(int j = 0; j < vWidth_; j++)
        {
            vec_ = cv::Vec3f((*src_)[0]/255.0, (*src_)[1]/255.0, (*src_)[2]/255.0);//Please note that OpenCV store pixels as BGR.

            vImage_.at<cv::Vec3f>(vHeight_-1-i, j) = vec_;

            ++src_;
        }
}

if(! vImage_.data ) // Check for invalid input
    printf("failed to read image by OpenCV.");
else
{
    cv::namedWindow( windowName_, CV_WINDOW_AUTOSIZE);
    cv::imshow( windowName_, vImage_); // Show the image.
}

Try the following:

cv::Mat image = ...do some stuff...;

image.at<cv::Vec3b>(y,x); gives you the RGB (it might be ordered as BGR) vector of type cv::Vec3b

image.at<cv::Vec3b>(y,x)[0] = newval[0];
image.at<cv::Vec3b>(y,x)[1] = newval[1];
image.at<cv::Vec3b>(y,x)[2] = newval[2];

The low-level way would be to access the matrix data directly. In an RGB image (which I believe OpenCV typically stores as BGR), and assuming your cv::Mat variable is called frame, you could get the blue value at location (x, y) (from the top left) this way:

frame.data[frame.channels()*(frame.cols*y + x)];

Likewise, to get B, G, and R:

uchar b = frame.data[frame.channels()*(frame.cols*y + x) + 0];    
uchar g = frame.data[frame.channels()*(frame.cols*y + x) + 1];
uchar r = frame.data[frame.channels()*(frame.cols*y + x) + 2];

Note that this code assumes the stride is equal to the width of the image.