How can I read lines from the end of file in Perl?

In *nix, you can use the tail command.

tail -1000 yourfile | perl ...

That will write only the last 1000 lines to the perl program.

On Windows, there are gnuwin32 and unxutils packages both have tail utility.

The File::ReadBackwards module allows you to read a file in reverse order. This makes it easy to get the last N lines as long as you aren't order dependent. If you are and the needed data is small enough (which it should be in your case) you could read the last 1000 lines into an array and then reverse it.