How write csv file without new line character in last line?

Use file.seek to move file pointer before the last \r\n, then use file.truncate.

import os
import csv

with open('eggs.csv', 'wb') as csvfile:
    spamwriter = csv.writer(csvfile, delimiter=' ',
                            quotechar='|', quoting=csv.QUOTE_MINIMAL)
    spamwriter.writerow(['Spam'] * 5 + ['Baked Beans'])
    spamwriter.writerow(['Spam', 'Lovely Spam', 'Wonderful Spam'])
    csvfile.seek(-2, os.SEEK_END) # <---- 2 : len('\r\n')
    csvfile.truncate()            # <----

NOTE: You should change -2 if you use different lineterminator. I used -2 because \r\n is default lineterminator.

here is a solution that removes the newline symbols from last line of csv, using rstrip:

def remove_last_line_from_csv(filename):
    with open(filename) as myFile:
        lines = myFile.readlines()
        last_line = lines[len(lines)-1]
        lines[len(lines)-1] = last_line.rstrip()
    with open(filename, 'w') as myFile:    
        myFile.writelines(lines)