Reload collection
To clear a collection you can call:
$this->_myCollection->clear();
To reload a collection you can call:
$this->_myCollection->load();
If you applied filters, you can reset collection with :
$this->_myCollection->clear()->getSelect()->reset(\Zend_Db_Select::WHERE);
Then :
$this->_myCollection->addFieldToFilter('field', $singleItems->getSomething());
$this->_myCollection->load();