Remove Dollar Sign from Entire Python Pandas Dataframe

You need escape $ by \:

dftest[colstocheck] = dftest[colstocheck].replace({'\$':''}, regex = True)
print (dftest)
   A  B        C    D  E  F
0  1  4       f;   s%  5  7
1  2  5       d:   d;  3  4
2  3  6  sda%;sd  d;p  6  3