Is it possible to define more than one function per file in MATLAB, and access them from outside that file?

The first function in an m-file (i.e. the main function), is invoked when that m-file is called. It is not required that the main function have the same name as the m-file, but for clarity it should. When the function and file name differ, the file name must be used to call the main function.

All subsequent functions in the m-file, called local functions (or "subfunctions" in the older terminology), can only be called by the main function and other local functions in that m-file. Functions in other m-files can not call them. Starting in R2016b, you can add local functions to scripts as well, although the scoping behavior is still the same (i.e. they can only be called from within the script).

In addition, you can also declare functions within other functions. These are called nested functions, and these can only be called from within the function they are nested. They can also have access to variables in functions in which they are nested, which makes them quite useful albeit slightly tricky to work with.

More food for thought...

There are some ways around the normal function scoping behavior outlined above, such as passing function handles as output arguments as mentioned in the answers from SCFrench and Jonas (which, starting in R2013b, is facilitated by the localfunctions function). However, I wouldn't suggest making it a habit of resorting to such tricks, as there are likely much better options for organizing your functions and files.

For example, let's say you have a main function A in an m-file A.m, along with local functions D, E, and F. Now let's say you have two other related functions B and C in m-files B.m and C.m, respectively, that you also want to be able to call D, E, and F. Here are some options you have:

• Put D, E, and F each in their own separate m-files, allowing any other function to call them. The downside is that the scope of these functions is large and isn't restricted to just A, B, and C, but the upside is that this is quite simple.

• Create a defineMyFunctions m-file (like in Jonas' example) with D, E, and F as local functions and a main function that simply returns function handles to them. This allows you to keep D, E, and F in the same file, but it doesn't do anything regarding the scope of these functions since any function that can call defineMyFunctions can invoke them. You also then have to worry about passing the function handles around as arguments to make sure you have them where you need them.

• Copy D, E and F into B.m and C.m as local functions. This limits the scope of their usage to just A, B, and C, but makes updating and maintenance of your code a nightmare because you have three copies of the same code in different places.

• Use private functions! If you have A, B, and C in the same directory, you can create a subdirectory called private and place D, E, and F in there, each as a separate m-file. This limits their scope so they can only be called by functions in the directory immediately above (i.e. A, B, and C) and keeps them together in the same place (but still different m-files):

  myDirectory/
      A.m
      B.m
      C.m
      private/
          D.m
          E.m
          F.m
  

All this goes somewhat outside the scope of your question, and is probably more detail than you need, but I thought it might be good to touch upon the more general concern of organizing all of your m-files. ;)

Generally, the answer to your question is no, you cannot define more than one externally visible function per file. You can return function handles to local functions, though, and a convenient way to do so is to make them fields of a struct. Here is an example:

function funs = makefuns
  funs.fun1=@fun1;
  funs.fun2=@fun2;
end

function y=fun1(x)
  y=x;
end

function z=fun2
  z=1;
end

And here is how it could be used:

>> myfuns = makefuns;
>> myfuns.fun1(5)    
ans =
     5
>> myfuns.fun2()     
ans =
     1

The only way to have multiple, separately accessible functions in a single file is to define STATIC METHODS using object-oriented programming. You'd access the function as myClass.static1(), myClass.static2() etc.

OOP functionality is only officially supported since R2008a, so unless you want to use the old, undocumented OOP syntax, the answer for you is no, as explained by @gnovice.

EDIT

One more way to define multiple functions inside a file that are accessible from the outside is to create a function that returns multiple function handles. In other words, you'd call your defining function as [fun1,fun2,fun3]=defineMyFunctions, after which you could use out1=fun1(inputs) etc.