Is it possible to get 0 by subtracting two unequal floating point numbers?

As a workaround, what about the following?

public double calculation(double a, double b) {
     double c = a - b;
     if (c == 0)
     {
         return 0;
     }
     else
     {
         return 2 / c;
     }
}

That way you don't depend on IEEE support in any language.

In Java, a - b is never equal to 0 if a != b. This is because Java mandates IEEE 754 floating point operations which support denormalized numbers. From the spec:

In particular, the Java programming language requires support of IEEE 754 denormalized floating-point numbers and gradual underflow, which make it easier to prove desirable properties of particular numerical algorithms. Floating-point operations do not "flush to zero" if the calculated result is a denormalized number.

If an FPU works with denormalized numbers, subtracting unequal numbers can never produce zero (unlike multiplication), also see this question.

For other languages, it depends. In C or C++, for example, IEEE 754 support is optional.

That said, it is possible for the expression 2 / (a - b) to overflow, for example with a = 5e-308 and b = 4e-308.