Is there a way to hide a specific column in a DataGrid when AutoGenerateColumns=True?

in your datagrid, subscribe for the AutoGeneratingColumn event, the event args (DataGridAutoGeneratingColumnEventArgs) has the column name and a "Cancel", if the column name is ID then set Cancel = true. should do the trick.

You can use a behavior (reusable code) to do the job... This way you can use attribute which would centralize the column visibility in one place.

Usage:

<Window
...
 xmlns:extension="clr-namespace:WpfControlLibrary.Extension;assembly=WpfControlLibrary">

<DataGrid ...
    extension:DataGridBehavior.UseBrowsableAttributeOnColumn="True">

...

public class YourObjectItem
{
    [Browsable(false)]
        public Assembly Assembly { get; set; }

Code:

using System;
using System.ComponentModel;
using System.Windows;
using System.Windows.Controls;

namespace HQ.Wpf.Util.Behaviors
{
    /// <summary>
    /// Using this behavior on a dataGRid will ensure to display only columns with "Browsable Attributes"
    /// </summary>
    public static class DataGridBehavior
    {
        public static readonly DependencyProperty UseBrowsableAttributeOnColumnProperty =
            DependencyProperty.RegisterAttached("UseBrowsableAttributeOnColumn",
            typeof(bool),
            typeof(DataGridBehavior),
            new UIPropertyMetadata(false, UseBrowsableAttributeOnColumnChanged));

        public static bool GetUseBrowsableAttributeOnColumn(DependencyObject obj)
        {
            return (bool)obj.GetValue(UseBrowsableAttributeOnColumnProperty);
        }

        public static void SetUseBrowsableAttributeOnColumn(DependencyObject obj, bool val)
        {
            obj.SetValue(UseBrowsableAttributeOnColumnProperty, val);
        }

        private static void UseBrowsableAttributeOnColumnChanged(DependencyObject obj, DependencyPropertyChangedEventArgs e)
        {
            var dataGrid = obj as DataGrid;
            if (dataGrid != null)
            {
                if ((bool)e.NewValue)
                {
                    dataGrid.AutoGeneratingColumn += DataGridOnAutoGeneratingColumn;
                }
                else
                {
                    dataGrid.AutoGeneratingColumn -= DataGridOnAutoGeneratingColumn;
                }
            }
        }

        private static void DataGridOnAutoGeneratingColumn(object sender, DataGridAutoGeneratingColumnEventArgs e)
        {
            var propDesc = e.PropertyDescriptor as PropertyDescriptor;

            if (propDesc != null)
            {
                foreach (Attribute att in propDesc.Attributes)
                {
                    var browsableAttribute = att as BrowsableAttribute;
                    if (browsableAttribute != null)
                    {
                        if (!browsableAttribute.Browsable)
                        {
                            e.Cancel = true;
                        }
                    }

                    // As proposed by "dba" stackoverflow user on webpage: 
                    // https://stackoverflow.com/questions/4000132/is-there-a-way-to-hide-a-specific-column-in-a-datagrid-when-autogeneratecolumns
                    // I added few next lines:
                    var displayName = att as DisplayNameAttribute;
                    if (displayName != null)
                    {
                        e.Column.Header = displayName.DisplayName;
                    }
                }
            }
        }
    }
}

Other possibility would be Visibility.Collapsed:

    private void dataGrid_AutoGeneratingColumn(object sender, 
        DataGridAutoGeneratingColumnEventArgs e)
    {
        //Set properties on the columns during auto-generation 
        switch (e.Column.Header.ToString())
        {
            case "rownameYouWantToHide":
                e.Column.Visibility = Visibility.Collapsed;
                break;
        }
    }