pygame make color transparent code example
Example 1: pygame draw transparent rectangle
s = pygame.Surface((1000,750)) # the size of your rect
s.set_alpha(128) # alpha level
s.fill((255,255,255)) # this fills the entire surface
windowSurface.blit(s, (0,0)) # (0,0) are the top-left coordinates
Example 2: how to set background color of an image to transparent in pygame
image = pygame.image.load("Your file location")
image.set_colorkey((0, 0, 0)) # If your bg color is black for the image