Remove leading and trailing spaces?

You can use the strip() to remove trailing and leading spaces.

>>> s = '   abd cde   '
>>> s.strip()
'abd cde'

Note: the internal spaces are preserved

Expand your one liner into multiple lines. Then it becomes easy:

f.write(re.split("Tech ID:|Name:|Account #:",line)[-1])

parts = re.split("Tech ID:|Name:|Account #:",line)
wanted_part = parts[-1]
wanted_part_stripped = wanted_part.strip()
f.write(wanted_part_stripped)

Should be noted that strip() method would trim any leading and trailing whitespace characters from the string (if there is no passed-in argument). If you want to trim space character(s), while keeping the others (like newline), this answer might be helpful:

sample = '  some string\n'
sample_modified = sample.strip(' ')

print(sample_modified)  # will print 'some string\n'

strip([chars]): You can pass in optional characters to strip([chars]) method. Python will look for occurrences of these characters and trim the given string accordingly.