Signalr/Hub not loading in IIS 7 but working correctly in Visual Studio

The problem was solved by setting the following flags in the web.config.

<configuration>
    <system.webServer>
        <validation validateIntegratedModeConfiguration="false" />
        <modules runAllManagedModulesForAllRequests="true">
        </modules>
    </system.webServer>
</configuration>

For some reason Nuget did not set these values for Elmah or SignalR

I was facing a similar issue, I just changed the /signalr/hubs to /virtualDirectoryName/signalr/hubs and it worked.

Replace:

<script src="/signalr/hubs" type="text/javascript"></script>

with:

<script src="<%= ResolveUrl("~/signalr/hubs") %>" type="text/javascript"></script>