stack all levels of a MultiIndex

You can first find len of levels, get range and pass it to stack:

print (df.columns.nlevels)
3

print (list(range(df.columns.nlevels)))
[0, 1, 2]

print (df.stack(list(range(df.columns.nlevels))))
0  a  A  One     0
         Two     1
      B  One     2
         Two     3
   b  A  One     4
         Two     5
      B  One     6
         Two     7
1  a  A  One     8
         Two     9
      B  One    10
         Two    11
   b  A  One    12
         Two    13
      B  One    14
         Two    15
dtype: int32