C# Only One Thread Executes

You can use a lock object in combination with Monitor.TryEnter.

private Object outputLock = new Object();

public void setOutput(int value)
{
    if Monitor.TryEnter(outputLock)
    {
        try
        {
            .... your code in here
        }
        finally
        {
            Monitor.Exit(outputLock);
        }
    }
}

Only one thread at at time will be allowed into the Monitor.TryEnter block. If a thread arrives here while another thread is inside, then Monitor.TryEnter returns false.

You can use a Mutex

using System;
using System.Threading;

class Test
{
    // Create a new Mutex. The creating thread does not own the
    // Mutex.
    private static Mutex mut = new Mutex();
    private const int numIterations = 1;
    private const int numThreads = 3;

    static void Main()
    {
        // Create the threads that will use the protected resource.
        for(int i = 0; i < numThreads; i++)
        {
            Thread myThread = new Thread(new ThreadStart(MyThreadProc));
            myThread.Name = String.Format("Thread{0}", i + 1);
            myThread.Start();
        }

        // The main thread exits, but the application continues to
        // run until all foreground threads have exited.
    }

    private static void MyThreadProc()
    {
        for(int i = 0; i < numIterations; i++)
        {
            UseResource();
        }
    }

    // This method represents a resource that must be synchronized
    // so that only one thread at a time can enter.
    private static void UseResource()
    {
        // Wait until it is safe to enter.
        mut.WaitOne();

        Console.WriteLine("{0} has entered the protected area", 
            Thread.CurrentThread.Name);

        // Place code to access non-reentrant resources here.

        // Simulate some work.
        Thread.Sleep(500);

        Console.WriteLine("{0} is leaving the protected area\r\n", 
            Thread.CurrentThread.Name);

        // Release the Mutex.
        mut.ReleaseMutex();
    }
}