How can I assign the F5 key to be refresh in Chrome on Mac OS X?

You can change the shortcuts of menu items in System Preferences, but you can't assign multiple shortcuts to them.

You could also assign a shortcut to a script like this:

try
    tell application "Google Chrome" to reload tab 1 of window 1
end try

1) Go to Preferences > Keyboard > Keyboard Shortcuts.

2) Select "Application Shortcuts" from the left menu.

3) Click "+".

4) Fill out the form like so: Application Google Chrome, Menu Title: Reload This Page, Keyboard Shortcut F5.

5) Click "Add".

Looks like they moved the location of the shortcut at least since the previous commenter posted.

I'm using El Capitan 10.11.13 and for me it is in System Preferences > Keyboard Shortcuts > App Shortcuts

Screenshot

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