How to check if a string in Python is in ASCII?

I think you are not asking the right question--

A string in python has no property corresponding to 'ascii', utf-8, or any other encoding. The source of your string (whether you read it from a file, input from a keyboard, etc.) may have encoded a unicode string in ascii to produce your string, but that's where you need to go for an answer.

Perhaps the question you can ask is: "Is this string the result of encoding a unicode string in ascii?" -- This you can answer by trying:

try:
    mystring.decode('ascii')
except UnicodeDecodeError:
    print "it was not a ascii-encoded unicode string"
else:
    print "It may have been an ascii-encoded unicode string"

def is_ascii(s):
    return all(ord(c) < 128 for c in s)