seaborn heatmap using pandas dataframe

Use pandas.DataFrame.pivot (no aggregation of values=) or pandas.DataFrame.pivot_table (with aggregation of values=) to reshape the dataframe from a long to wide form. The index will be on the y-axis, and the columns will be on the x-axis. See Reshaping and pivot tables for an overview.

In [96]: result
Out[96]: 
MutProb               0.001      0.005      0.010     0.050     0.100
SymmetricDivision                                                    
0.2               -6.146121  -8.571063  -9.784686 -6.051482 -0.964818
0.4               -6.473629  -8.936463  -9.455776 -6.885229 -0.652147
0.6               -6.760559  -9.292469  -9.551801 -6.621639 -0.392256
0.8               -7.196407  -9.544065 -10.536340 -6.996394 -0.722602
1.0               -8.027475 -10.502450 -11.408114 -9.175349 -4.180864

Then you can pass the 2D array (or DataFrame) to seaborn.heatmap or plt.pcolor:

import pandas as pd
import seaborn as sns
import matplotlib.pyplot as plt

# load the sample data
df = pd.DataFrame({'MutProb': [0.1,
  0.05, 0.01, 0.005, 0.001, 0.1, 0.05, 0.01, 0.005, 0.001, 0.1, 0.05, 0.01, 0.005, 0.001, 0.1, 0.05, 0.01, 0.005, 0.001, 0.1, 0.05, 0.01, 0.005, 0.001], 'SymmetricDivision': [1.0, 1.0, 1.0, 1.0, 1.0, 0.8, 0.8, 0.8, 0.8, 0.8, 0.6, 0.6, 0.6, 0.6, 0.6, 0.4, 0.4, 0.4, 0.4, 0.4, 0.2, 0.2, 0.2, 0.2, 0.2], 'test': ['sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule', 'sackin_yule'], 'value': [-4.1808639999999997, -9.1753490000000006, -11.408113999999999, -10.50245, -8.0274750000000008, -0.72260200000000008, -6.9963940000000004, -10.536339999999999, -9.5440649999999998, -7.1964070000000007, -0.39225599999999999, -6.6216390000000001, -9.5518009999999993, -9.2924690000000005, -6.7605589999999998, -0.65214700000000003, -6.8852289999999989, -9.4557760000000002, -8.9364629999999998, -6.4736289999999999, -0.96481800000000006, -6.051482, -9.7846860000000007, -8.5710630000000005, -6.1461209999999999]})

# pivot the dataframe from long to wide form
result = df.pivot(index='SymmetricDivision', columns='MutProb', values='value')

sns.heatmap(result, annot=True, fmt="g", cmap='viridis')
plt.show()

yields

Another option using .grouby() and .unstack()

df_m = df.groupby(["SymmetricDivision", "MutProb"])
          .size()
          .unstack(level=0)

sns.heatmap(df_m)