How to select rows with NaN in particular column?

Try the following:

df[df['Col2'].isnull()]

@qbzenker provided the most idiomatic method IMO

Here are a few alternatives:

In [28]: df.query('Col2 != Col2') # Using the fact that: np.nan != np.nan
Out[28]:
   Col1  Col2  Col3
1     0   NaN   0.0

In [29]: df[np.isnan(df.Col2)]
Out[29]:
   Col1  Col2  Col3
1     0   NaN   0.0