How to stop creating .DS_Store on Mac?

Please install http://asepsis.binaryage.com/ and then reboot your mac.

ASEPSIS redirect all .DS_Store on your mac to /usr/local/.dscage

After that, You could recursively delete all .DS_Store from your Mac.

find ~ -name ".DS_Store" -delete
or
find <your path> -name ".DS_Store" -delete

You should repeat procedure after each major Mac update.

Its is possible by using mach_inject. Take a look at Death to .DS_Store

I found that overriding HFSPlusPropertyStore::FlushChanges() with a function that simply did nothing, successfully prevented the creation of .DS_Store files on both Snow Leopard and Lion.

DeathToDSStore source code

NOTE: On 10.11 you can not inject code into system apps.

NOTE: "Asepsis is no longer under active development and supported under OS X 10.11 (El Capitan) and later."

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Here's a comprehensive review of your options. Asepsis (the second solution mentioned) seems to be what you're looking for, it re-routes .DS_Store creation to a unified cache instead of being located on every folder.